Engle Laboratory
Techniques
DRG explant labeled with anti-tubulin, L. Jia (confocal)
- Phenotypic characterization of mouse models of disease
- DTI and DSI MR of adult and embryonic mouse brains
- Analysis of isolated embryonic neurons from hippocampus, cortex, spinal cord, oculomotor anlage
- Growth assays of explant cultures of DRG, embryonic spinal cord, midbrain & hindbrain
- Transfection, electroporation and siRNA treatment of neurons & other cells
- Microscopy: confocal, deconvolution, EM
- Morphometrics; kymography
- DiO, DiI multispectrum anterograde & retrograde labeling of axonal tracks
- Live cell imaging of kinesin motility & microtubule dynamics
- Ultracentrifugal methods: cell fractionation, sucrose gradient separation of protein complexes
- Microtubule biochemical assays
- Immunoprecipitation, epitope-based pull downs & immunoblotting
- Affinity chromatography protein purification
- Mass spec proteomics for identification of protein partners
- Native and SDS gel electrophoresis
- Cell signaling assays
- Real-time RT-PCR
- Mutation analysis: DNA sequencing, WAVE analysis, genome-wide screens (GWS)
