Publications | Overview
Barrachina, M.N., Pernes, G., Becker, I.C. et al. Efficient megakaryopoiesis and platelet production require phospholipid remodeling and PUFA uptake through CD36. Nat Cardiovasc Res (2023). https://doi.org/10.1038/s44161-023-00305-y
Tilburg, J., Stone, A., Billingsley, J., Scoville, D., Pavenko, A., Liang, Y., ... & Machlus, K. (2023). Spatial transcriptomics of murine bone marrow megakaryocytes at single cell resolution. Research and Practice in Thrombosis and Haemostasis, 100158.
Khan, A. O., Rodriguez-Romera, A., Reyat, J. S., Olijnik, A. A., Colombo, M., Wang, G., ... & Psaila, B. (2023). Human bone marrow organoids for disease modeling, discovery, and validation of therapeutic targets in hematologic malignancies. Cancer discovery, 13(2), 364-385.
Tilburg, J., Becker, I. C., & Italiano, J. E. (2022). Don’t you forget about me (gakaryocytes). Blood, 139(22), 3245-3254.
Stone, A. P., Nikols, E., Freire, D., & Machlus, K. R. (2022). The pathobiology of platelet and megakaryocyte extracellular vesicles: A (c)lot has changed. Journal of thrombosis and haemostasis : JTH, 20(7), 1550–1558. https://doi.org/10.1111/jth.15750
Becker, I. C., & Italiano, J. E. (2022). The triple crown of platelet generation. Blood, The Journal of the American Society of Hematology, 139(14), 2100-2101.
Slingsby, M. H. L., Vijey, P., Tsai, I. T., Roweth, H., Couldwell, G., Wilkie, A. R., ... & Italiano, J. J. E. (2022). Sequence-specific 2'-O-methoxyethyl antisense oligonucleotides activate human platelets through glycoprotein VI, triggering formation of platelet-leukocyte aggregates. haematologica, 107(2), 519.
Boilard E, Machlus KR. Location is everything when it comes to megakaryocyte function. J Clin Invest. 2021 Jan 4;131(1):e144964.
Evans AL, Dalby A, Foster HR, Howard D, Waller AK, Taimoor M, Lawrence M, Mookerjee S, Lehmann M, Burton A, Valdez J, Thon J, Italiano J, Moreau T, Ghevaert C. Transfer to the clinic: refining forward programming of hPSCs to megakaryocytes for platelet production in bioreactors. Blood Adv. 2021 Apr 13;5(7):1977-1990.
Slingsby MHL, Vijey P, Tsai IT, Roweth H, Couldwell G, Wilkie AR, Gaus H, Goolsby JM, Okazaki R, Terkovich BE, Semple JW, Thon JN, Henry SP, Narayanan P, Italiano JE Jr. Sequence-specific 2'-O-methoxyethyl antisense oligonucleotides activate human platelets through glycoprotein VI, triggering formation of platelet-leukocyte aggregates. Haematologica. 2021 Feb 11. doi: 10.3324/haematol.2020.260059.
Puhm F, Boilard E, Machlus KR. Platelet Extracellular Vesicles: Beyond the Blood. Arterioscler Thromb Vasc Biol. 2021 Jan;41(1):87-96.
Khan AO, Stapley RJ, Pike JA, Wijesinghe SN, Reyat JS, Almazni I, Machlus KR, Morgan NV; UK GAPP Study Group. Novel gene variants in patients with platelet-based bleeding using combined exome sequencing and RNAseq murine expression data. J Thromb Haemost. 2021 Jan;19(1):262-268.
Matsuura S, Thompson CR, Belghasem ME, Bekendam RH, Piasecki A, Leiva O, Ray A, Italiano J, Yang M, Merill-Skoloff G, Chitalia VC, Flaumenhaft R, Ravid K. Platelet Dysfunction and Thrombosis in JAK2V617F-Mutated Primary Myelofibrotic Mice.Arterioscler Thromb Vasc Biol. 2020 Oct;40(10):e262-e272.
Butov KR, Osipova EY, Mikhalkin NB, Trubina NM, Panteleev MA, Machlus KR. Platelets. 2020 Sep 16:1-8. In vitro megakaryocyte culture from human bone marrow aspirates as a research and diagnostic tool.
French SL, Vijey P, Karhohs KW, Wilkie AR, Horin LJ, Ray A, Posorske B, Carpenter AE, Machlus KR, Italiano JE Jr. High-content, label-free analysis of proplatelet production from megakaryocytes. J Thromb Haemost. 2020 Oct;18(10):2701-2711.
French SL, Butov KR, Allaeys I, Canas J, Morad G, Davenport P, Laroche A, Trubina NM, Italiano JE, Moses MA, Sola-Visner M, Boilard E, Panteleev MA, Machlus KR. Platelet-derived extracellular vesicles infiltrate and modify the bone marrow during inflammation. Blood Adv. 2020 Jul 14;4(13):3011-3023.
Asquith NL, Italiano JE. Sniffing out the aroma(tase) of drug-induced thrombocytopenia. Blood. 2020 Jun 11;135(24):2116-2117.
Noetzli LJ, French SL, Machlus KR. New Insights Into the Differentiation of Megakaryocytes From Hematopoietic Progenitors. Arterioscler Thromb Vasc Biol. 2019 Jul; 39(7):1288-1300.
Couldwell G, Machlus KR. Modulation of megakaryopoiesis and platelet production during inflammation. Thromb Res. 2019 Jul; 179:114-120.
Johnson KE, Ceglowski JR, Roweth HG, Forward JA, Tippy MD, El-Husayni S, Kulenthirarajan R, Malloy MW, Machlus KR, Chen WY, Italiano JE, Battinelli EM. Aspirin inhibits platelets from reprogramming breast tumor cells and promoting metastasis. Blood Adv. 2019 Jan 22; 3(2):198-211.
Vijey P, Posorske B, Machlus KR. In vitro culture of murine megakaryocytes from fetal liver-derived hematopoietic stem cells. Platelets. 2018 Sep; 29(6):583-588. PMID: 30047825.
Roweth HG, Parvin S, Machlus KR. Megakaryocyte modification of platelets in thrombocytopenia. Curr Opin Hematol. 2018 Sep; 25(5):410-415. PMID: 29985173.
Takagi S, Tsukamoto S, Park J, Johnson KE, Kawano Y, Moschetta M, Liu CJ, Mishima Y, Kokubun K, Manier S, Salem KZ, Huynh D, Sacco A, Forward J, Roccaro AM, Battinelli EM, Ghobrial IM. Platelets Enhance Multiple Myeloma Progression via IL-1ß Upregulation. Clin Cancer Res. 2018 May 15; 24(10):2430-2439. PMID: 29440174.
Cloutier N, Allaeys I, Marcoux G, Machlus KR, Mailhot B, Zufferey A, Levesque T, Becker Y, Tessandier N, Melki I, Zhi H, Poirier G, Rondina MT, Italiano JE, Flamand L, McKenzie SE, Cote F, Nieswandt B, Khan WI, Flick MJ, Newman PJ, Lacroix S, Fortin PR, Boilard E. Platelets release pathogenic serotonin and return to circulation after immune complex-mediated sequestration. Proc Natl Acad Sci U S A. 2018 02 13; 115(7):E1550-E1559. PMID: 29386381.
Zufferey A, Speck ER, Machlus KR, Aslam R, Guo L, McVey MJ, Kim M, Kapur R, Boilard E, Italiano JE, Semple JW. Mature murine megakaryocytes present antigen-MHC class I molecules to T cells and transfer them to platelets. Blood Adv. 2017 Sep 12; 1(20):1773-1785. PMID: 29296823.
Paul DS, Casari C, Wu C, Piatt R, Pasala S, Campbell RA, Poe KO, Ghalloussi D, Lee RH, Rotty JD, Cooley BC, Machlus KR, Italiano JE, Weyrich AS, Bear JE, Bergmeier W. Deletion of the Arp2/3 complex in megakaryocytes leads to microthrombocytopenia in mice. Blood Adv. 2017 Aug 08; 1(18):1398-1408. PMID: 29104956.
Machlus KR, Wu SK, Vijey P, Soussou TS, Liu ZJ, Shacham E, Unger TJ, Kashyap T, Klebanov B, Sola-Visner M, Crochiere M, Italiano JE, Landesman Y. Selinexor-induced thrombocytopenia results from inhibition of thrombopoietin signaling in early megakaryopoiesis. Blood. 2017 08 31; 130(9):1132-1143. PMID: 28630120.
Johnson KE, Forward JA, Tippy MD, Ceglowski JR, El-Husayni S, Kulenthirarajan R, Machlus KR, Mayer EL, Italiano JE, Battinelli EM. Tamoxifen Directly Inhibits Platelet Angiogenic Potential and Platelet-Mediated Metastasis. Arterioscler Thromb Vasc Biol. 2017 04; 37(4):664-674. PMID: 28153880.
Kim K, Tseng A, Barazia A, Italiano JE, Cho J. DREAM plays an important role in platelet activation and thrombogenesis. Blood. 2017 01 12; 129(2):209-225. PMID: 27903531.
Machlus KR, Wu SK, Stumpo DJ, Soussou TS, Paul DS, Campbell RA, Kalwa H, Michel T, Bergmeier W, Weyrich AS, Blackshear PJ, Hartwig JH, Italiano JE. Synthesis and dephosphorylation of MARCKS in the late stages of megakaryocyte maturation drive proplatelet formation. Blood. 2016 Mar 17; 127(11):1468-80. PMID: 26744461. View in PubMed
Machlus KR, Johnson KE, Kulenthirarajan R, Forward JA, Tippy MD, Soussou TS, El-Husayni SH, Wu SK, Wang S, Watnick RS, Italiano JE, Battinelli EM. CCL5 derived from platelets increases megakaryocyte proplatelet formation. Blood. 2016 Feb 18; 127(7):921-6. PMID: 26647394. View in PubMed
Carubbi C, Masselli E, Martini S, Galli D, Aversa F, Mirandola P, Italiano JE, Gobbi G, Vitale M. Human thrombopoiesis depends on Protein kinase Cd/protein kinase Ce functional couple. Haematologica. 2016 07; 101(7):812-20. PMID: 27081176; PMCID: PMC5004460. View in PubMed
Matsuura S, Mi R, Koupenova M, Eliades A, Patterson S, Toselli P, Thon J, Italiano JE, Trackman PC, Papadantonakis N, Ravid K. Lysyl oxidase is associated with increased thrombosis and platelet reactivity. Blood. 2016 Mar 17; 127(11):1493-501. PMID: 26755713; PMCID: PMC4797025 [Available on 03/17/17]. View in PubMed